Abstract 127: A Mouse Model of Mandibular Distraction Osteogenesis
نویسندگان
چکیده
METHODS: Human umbilical cords were harvested following routine delivery and WJ was isolated and purified. In vitro, WJ derived stem cells were placed in osteogenic differentiation medium for 14 days, followed by Alizarin Red S staining to evaluate mineral deposition. In vivo, we used a rat critical-size alveolar bone defect model to investigate the use of Wharton’s Jelly (WJ) in formation of bone. WJ was implanted into a critical size (7 x 4 x 3 mm) alveolar bone defect model representative of cleft palate surgery in 10–11 week old male Sprague-dawley rats. The defects were monitored weekly with CT imaging of living animals to evaluate bone formation in time, followed by histology evaluation at week 24.
منابع مشابه
Effect of strontium citrate on bone consolidation during mandibular distraction osteogenesis.
OBJECTIVES/HYPOTHESIS Mandibular distraction osteogenesis (MDO) involves a lengthy consolidation phase where complications can occur. Strontium is an element that has been shown to improve bone healing. The objective of this study was to determine whether strontium citrate can be used to enhance bone healing during MDO in a rabbit model. STUDY DESIGN Prospective animal model study. METHODS ...
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